A simple mix-and-read bacteria detection system based on a DNAzyme and a molecular beacon

Ting Cao; Yongcheng Wang; Ling Li Zhao; Ye Wang; Ye Tao; John A. Heyman; David A. Weitz; Ying Lin Zhou; Xin Xiang Zhang

doi:10.1039/c9cc03746b

A simple mix-and-read bacteria detection system based on a DNAzyme and a molecular beacon

Ting Cao
, Yongcheng Wang
, Ling Li Zhao
, Ye Wang
, Ye Tao
, John A. Heyman^*
, David A. Weitz
, Ying Lin Zhou
, Xin Xiang Zhang

^*Corresponding author for this work

Peking University
Harvard University
Wyss Institute for Biologically Inspired Engineering

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

Here, we describe a simple mix-and-read method for the detection of specific bacterial strains that uses a DNAzyme and a molecular beacon to generate a signal. We have greatly improved upon the previously described DNAzyme-based bacteria detection method by eliminating a tedious preparation step while maintaining detection sensitivity.

Original language	English
Pages (from-to)	7358-7361
Number of pages	4
Journal	Chemical Communications
Volume	55
Issue number	51
DOIs	https://doi.org/10.1039/c9cc03746b
State	Published - 2019
Externally published	Yes

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title = "A simple mix-and-read bacteria detection system based on a DNAzyme and a molecular beacon",

abstract = "Here, we describe a simple mix-and-read method for the detection of specific bacterial strains that uses a DNAzyme and a molecular beacon to generate a signal. We have greatly improved upon the previously described DNAzyme-based bacteria detection method by eliminating a tedious preparation step while maintaining detection sensitivity.",

author = "Ting Cao and Yongcheng Wang and Zhao, \{Ling Li\} and Ye Wang and Ye Tao and Heyman, \{John A.\} and Weitz, \{David A.\} and Zhou, \{Ying Lin\} and Zhang, \{Xin Xiang\}",

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doi = "10.1039/c9cc03746b",

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T1 - A simple mix-and-read bacteria detection system based on a DNAzyme and a molecular beacon

AU - Cao, Ting

AU - Wang, Yongcheng

AU - Zhao, Ling Li

AU - Wang, Ye

AU - Tao, Ye

AU - Heyman, John A.

AU - Weitz, David A.

AU - Zhou, Ying Lin

AU - Zhang, Xin Xiang

PY - 2019

Y1 - 2019

N2 - Here, we describe a simple mix-and-read method for the detection of specific bacterial strains that uses a DNAzyme and a molecular beacon to generate a signal. We have greatly improved upon the previously described DNAzyme-based bacteria detection method by eliminating a tedious preparation step while maintaining detection sensitivity.

AB - Here, we describe a simple mix-and-read method for the detection of specific bacterial strains that uses a DNAzyme and a molecular beacon to generate a signal. We have greatly improved upon the previously described DNAzyme-based bacteria detection method by eliminating a tedious preparation step while maintaining detection sensitivity.

UR - https://www.scopus.com/pages/publications/85067661412

U2 - 10.1039/c9cc03746b

DO - 10.1039/c9cc03746b

M3 - 文章

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AN - SCOPUS:85067661412

SN - 1359-7345

VL - 55

SP - 7358

EP - 7361

JO - Chemical Communications

JF - Chemical Communications

IS - 51

ER -

A simple mix-and-read bacteria detection system based on a DNAzyme and a molecular beacon

Abstract

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