A simple and sensitive coumarin-based fluorescence probe (ZXD-1) for determination of hydrogen peroxide and its application in bioimaging

Xiaodan Zeng; Shihua Yu; Zhigang Liu; Mingshuo Ma; Jie Chen

doi:10.1016/j.molstruc.2023.137124

A simple and sensitive coumarin-based fluorescence probe (ZXD-1) for determination of hydrogen peroxide and its application in bioimaging

Xiaodan Zeng^*
, Shihua Yu
, Zhigang Liu
, Mingshuo Ma
, Jie Chen

^*Corresponding author for this work

Jilin Institute of Chemical Technology

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

A novel coumarin-based fluorescence probe (ZXD-1) was synthesized for detecting hydrogen peroxide (H₂O₂). The assay is carried out through the fluorescence recovery of the probe by the breaking of easter group in the presence of H₂O₂. And the degree of fluorescence increase of the probe at 455 nm is linearly related to the concentration in the range of 0–50 μM. The limit of detection (LOD) for H₂O₂ is 0.18 μM. This method was proved to be with excellent specificity and sensitivity. This probe was further used to image RAW264.7 macrophage cells and zebrafish based on the specifical response to H₂O₂, which showed its good cell-membrane permeability and its potential ability of detecting H₂O₂ in living organisms. Moreover, it can be successfully applied for detecting H₂O₂ in real water samples.

Original language	English
Article number	137124
Journal	Journal of Molecular Structure
Volume	1299
DOIs	https://doi.org/10.1016/j.molstruc.2023.137124
State	Published - 5 Mar 2024
Externally published	Yes

Keywords

Coumarin
Fluorescence bioimaging
Fluorescent probe

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10.1016/j.molstruc.2023.137124

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title = "A simple and sensitive coumarin-based fluorescence probe (ZXD-1) for determination of hydrogen peroxide and its application in bioimaging",

abstract = "A novel coumarin-based fluorescence probe (ZXD-1) was synthesized for detecting hydrogen peroxide (H2O2). The assay is carried out through the fluorescence recovery of the probe by the breaking of easter group in the presence of H2O2. And the degree of fluorescence increase of the probe at 455 nm is linearly related to the concentration in the range of 0{\textendash}50 μM. The limit of detection (LOD) for H2O2 is 0.18 μM. This method was proved to be with excellent specificity and sensitivity. This probe was further used to image RAW264.7 macrophage cells and zebrafish based on the specifical response to H2O2, which showed its good cell-membrane permeability and its potential ability of detecting H2O2 in living organisms. Moreover, it can be successfully applied for detecting H2O2 in real water samples.",

keywords = "Coumarin, Fluorescence bioimaging, Fluorescent probe",

author = "Xiaodan Zeng and Shihua Yu and Zhigang Liu and Mingshuo Ma and Jie Chen",

note = "Publisher Copyright: {\textcopyright} 2023",

year = "2024",

month = mar,

day = "5",

doi = "10.1016/j.molstruc.2023.137124",

language = "英语",

volume = "1299",

journal = "Journal of Molecular Structure",

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TY - JOUR

T1 - A simple and sensitive coumarin-based fluorescence probe (ZXD-1) for determination of hydrogen peroxide and its application in bioimaging

AU - Zeng, Xiaodan

AU - Yu, Shihua

AU - Liu, Zhigang

AU - Ma, Mingshuo

AU - Chen, Jie

PY - 2024/3/5

Y1 - 2024/3/5

N2 - A novel coumarin-based fluorescence probe (ZXD-1) was synthesized for detecting hydrogen peroxide (H2O2). The assay is carried out through the fluorescence recovery of the probe by the breaking of easter group in the presence of H2O2. And the degree of fluorescence increase of the probe at 455 nm is linearly related to the concentration in the range of 0–50 μM. The limit of detection (LOD) for H2O2 is 0.18 μM. This method was proved to be with excellent specificity and sensitivity. This probe was further used to image RAW264.7 macrophage cells and zebrafish based on the specifical response to H2O2, which showed its good cell-membrane permeability and its potential ability of detecting H2O2 in living organisms. Moreover, it can be successfully applied for detecting H2O2 in real water samples.

AB - A novel coumarin-based fluorescence probe (ZXD-1) was synthesized for detecting hydrogen peroxide (H2O2). The assay is carried out through the fluorescence recovery of the probe by the breaking of easter group in the presence of H2O2. And the degree of fluorescence increase of the probe at 455 nm is linearly related to the concentration in the range of 0–50 μM. The limit of detection (LOD) for H2O2 is 0.18 μM. This method was proved to be with excellent specificity and sensitivity. This probe was further used to image RAW264.7 macrophage cells and zebrafish based on the specifical response to H2O2, which showed its good cell-membrane permeability and its potential ability of detecting H2O2 in living organisms. Moreover, it can be successfully applied for detecting H2O2 in real water samples.

KW - Coumarin

KW - Fluorescence bioimaging

KW - Fluorescent probe

UR - https://www.scopus.com/pages/publications/85179002994

U2 - 10.1016/j.molstruc.2023.137124

DO - 10.1016/j.molstruc.2023.137124

M3 - 文章

AN - SCOPUS:85179002994

SN - 0022-2860

VL - 1299

JO - Journal of Molecular Structure

JF - Journal of Molecular Structure

M1 - 137124

ER -

A simple and sensitive coumarin-based fluorescence probe (ZXD-1) for determination of hydrogen peroxide and its application in bioimaging

Abstract

Keywords

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