TY - GEN
T1 - A rapid quantitative detection method for anaerobic denitrifying bacteria(ADNB)
AU - Wei, Li
AU - Yang, Ji Xian
AU - Wang, Yan Jun
AU - Ma, Fang
AU - Wang, Qiang
AU - Lv, Xiao Lei
PY - 2009
Y1 - 2009
N2 - There was a long detection circle and higher cost in the quantitative detection of anaerobic deitrifying bacteria(ADNB) in the wastewater of bioreactor and oil field. To solve these issues, the ADNB-MPN-PCR method, combining polymerase chain reaction(PCR) technology with most probable number, was studied as a rapid quantitative detection method for ADNB. The bacterium solution directly used for PCR amplification was prepared from wastewater, which ensured the accuracy of quantification; the universal primers, ADNB341 and ADNB870, located at 16S rDNA genes of ADNB were designed, and the amplified fragment was 548bp; the reaction system and amplification conditions of PCR were also optimized. The results indicate that the order of magnitude of this method is two magnitudes higher than that of MPN-Gries, which truly represents the actual quantity of ADNB in wastewater. The whole procedure takes only 3-4 hours and the results of the detection are extremely stable, so the cost is reduced and ADNB-MPN-PCR can be applied in practical processes.
AB - There was a long detection circle and higher cost in the quantitative detection of anaerobic deitrifying bacteria(ADNB) in the wastewater of bioreactor and oil field. To solve these issues, the ADNB-MPN-PCR method, combining polymerase chain reaction(PCR) technology with most probable number, was studied as a rapid quantitative detection method for ADNB. The bacterium solution directly used for PCR amplification was prepared from wastewater, which ensured the accuracy of quantification; the universal primers, ADNB341 and ADNB870, located at 16S rDNA genes of ADNB were designed, and the amplified fragment was 548bp; the reaction system and amplification conditions of PCR were also optimized. The results indicate that the order of magnitude of this method is two magnitudes higher than that of MPN-Gries, which truly represents the actual quantity of ADNB in wastewater. The whole procedure takes only 3-4 hours and the results of the detection are extremely stable, so the cost is reduced and ADNB-MPN-PCR can be applied in practical processes.
KW - 16S rDNA
KW - ADNB-MPN- PCR assay
KW - Anaerobic deitrifying bacteria
KW - Bacterium solution preparation
KW - Quantitative detection
UR - https://www.scopus.com/pages/publications/72749096323
U2 - 10.1109/icbbe.2009.5162232
DO - 10.1109/icbbe.2009.5162232
M3 - 会议稿件
AN - SCOPUS:72749096323
SN - 9781424429028
T3 - 3rd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2009
BT - 3rd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2009
T2 - 3rd International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2009
Y2 - 11 June 2009 through 13 June 2009
ER -