A quick method for routine analysis of C18 trans fatty acids in non-hydrogenated edible vegetable oils by gas chromatography-mass spectrometry

M. Zhang; X. Yang; H. T. Zhao; A. J. Dong; J. Wang; G. Y. Liu; P. Wang; C. L. Cheng; H. Zhang

doi:10.1016/j.foodcont.2015.04.027

A quick method for routine analysis of C18 trans fatty acids in non-hydrogenated edible vegetable oils by gas chromatography-mass spectrometry

M. Zhang
, X. Yang^*
, H. T. Zhao
, A. J. Dong
, J. Wang
, G. Y. Liu
, P. Wang
, C. L. Cheng
, H. Zhang

^*Corresponding author for this work

Harbin Institute of Technology
Chinese Academy of Agricultural Sciences

Research output: Contribution to journal › Article › peer-review

42 Scopus citations

Abstract

A quick routine analysis has been established for quantitative determination of thirteen individual C18 trans fatty acids in both pressed and solvent extracted non-hydrogenated edible vegetable oils, using gas chromatography-mass spectrometer (GC-MS) equipped with HP-88 capillary column following potassium hydroxide/methanol (KOH/MeOH) methylation. Based on optimization and appraisal of the characteristics of GC-MS, thirteen C18 trans fatty acid methyl ester (FAME) standards were separated and verified in 23min, validation experiments were also conducted. The correlation coefficient was R²≥0.99 in the linear range of each FAME. At the low, medium and high fortification levels, recoveries fell within 90-119%. The relative standard deviations were below 9% for all 13 FAMEs. Low limits of detection (0.001-0.002g/100g) and quantification (0.002-0.007g/100g) were readily achieved. The content of C18 trans fatty acid isomers in pressed and solvent extracted oils were analyzed using this method.

Original language	English
Pages (from-to)	293-301
Number of pages	9
Journal	Food Control
Volume	57
DOIs	https://doi.org/10.1016/j.foodcont.2015.04.027
State	Published - 2015

Keywords

C18 trans fatty acid
GC-MS
Non-hydrogenated edible vegetable oil
Routine analysis

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10.1016/j.foodcont.2015.04.027

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@article{d9cfb00753634f9887ab787914440054,

title = "A quick method for routine analysis of C18 trans fatty acids in non-hydrogenated edible vegetable oils by gas chromatography-mass spectrometry",

abstract = "A quick routine analysis has been established for quantitative determination of thirteen individual C18 trans fatty acids in both pressed and solvent extracted non-hydrogenated edible vegetable oils, using gas chromatography-mass spectrometer (GC-MS) equipped with HP-88 capillary column following potassium hydroxide/methanol (KOH/MeOH) methylation. Based on optimization and appraisal of the characteristics of GC-MS, thirteen C18 trans fatty acid methyl ester (FAME) standards were separated and verified in 23min, validation experiments were also conducted. The correlation coefficient was R2≥0.99 in the linear range of each FAME. At the low, medium and high fortification levels, recoveries fell within 90-119\%. The relative standard deviations were below 9\% for all 13 FAMEs. Low limits of detection (0.001-0.002g/100g) and quantification (0.002-0.007g/100g) were readily achieved. The content of C18 trans fatty acid isomers in pressed and solvent extracted oils were analyzed using this method.",

keywords = "C18 trans fatty acid, GC-MS, Non-hydrogenated edible vegetable oil, Routine analysis",

author = "M. Zhang and X. Yang and Zhao, \{H. T.\} and Dong, \{A. J.\} and J. Wang and Liu, \{G. Y.\} and P. Wang and Cheng, \{C. L.\} and H. Zhang",

note = "Publisher Copyright: {\textcopyright} 2015 Elsevier Ltd.",

year = "2015",

doi = "10.1016/j.foodcont.2015.04.027",

language = "英语",

volume = "57",

pages = "293--301",

journal = "Food Control",

issn = "0956-7135",

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TY - JOUR

T1 - A quick method for routine analysis of C18 trans fatty acids in non-hydrogenated edible vegetable oils by gas chromatography-mass spectrometry

AU - Zhang, M.

AU - Yang, X.

AU - Zhao, H. T.

AU - Dong, A. J.

AU - Wang, J.

AU - Liu, G. Y.

AU - Wang, P.

AU - Cheng, C. L.

AU - Zhang, H.

PY - 2015

Y1 - 2015

N2 - A quick routine analysis has been established for quantitative determination of thirteen individual C18 trans fatty acids in both pressed and solvent extracted non-hydrogenated edible vegetable oils, using gas chromatography-mass spectrometer (GC-MS) equipped with HP-88 capillary column following potassium hydroxide/methanol (KOH/MeOH) methylation. Based on optimization and appraisal of the characteristics of GC-MS, thirteen C18 trans fatty acid methyl ester (FAME) standards were separated and verified in 23min, validation experiments were also conducted. The correlation coefficient was R2≥0.99 in the linear range of each FAME. At the low, medium and high fortification levels, recoveries fell within 90-119%. The relative standard deviations were below 9% for all 13 FAMEs. Low limits of detection (0.001-0.002g/100g) and quantification (0.002-0.007g/100g) were readily achieved. The content of C18 trans fatty acid isomers in pressed and solvent extracted oils were analyzed using this method.

AB - A quick routine analysis has been established for quantitative determination of thirteen individual C18 trans fatty acids in both pressed and solvent extracted non-hydrogenated edible vegetable oils, using gas chromatography-mass spectrometer (GC-MS) equipped with HP-88 capillary column following potassium hydroxide/methanol (KOH/MeOH) methylation. Based on optimization and appraisal of the characteristics of GC-MS, thirteen C18 trans fatty acid methyl ester (FAME) standards were separated and verified in 23min, validation experiments were also conducted. The correlation coefficient was R2≥0.99 in the linear range of each FAME. At the low, medium and high fortification levels, recoveries fell within 90-119%. The relative standard deviations were below 9% for all 13 FAMEs. Low limits of detection (0.001-0.002g/100g) and quantification (0.002-0.007g/100g) were readily achieved. The content of C18 trans fatty acid isomers in pressed and solvent extracted oils were analyzed using this method.

KW - C18 trans fatty acid

KW - GC-MS

KW - Non-hydrogenated edible vegetable oil

KW - Routine analysis

UR - https://www.scopus.com/pages/publications/84929074881

U2 - 10.1016/j.foodcont.2015.04.027

DO - 10.1016/j.foodcont.2015.04.027

M3 - 文章

AN - SCOPUS:84929074881

SN - 0956-7135

VL - 57

SP - 293

EP - 301

JO - Food Control

JF - Food Control

ER -

A quick method for routine analysis of C18 trans fatty acids in non-hydrogenated edible vegetable oils by gas chromatography-mass spectrometry

Abstract

Keywords

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