TY - GEN
T1 - A novel nanoliter liquid dispensing technology for protein crystallization
AU - Yaxin, Liu
AU - Liguo, Chen
AU - Lining, Sun
PY - 2007
Y1 - 2007
N2 - Recently, high-throughput protein crystallization requires the ability to transfer submicroliter volumes of sample with a large range of viscosities. In this paper, a solenoid-based non-contact liquid handling instrument with high-speed flow sensor was developed to meet this requirement. The flow sensor-driven dispenser with feedback control automatically compensates for encountered differences or changes in sample viscosity by adjusting the valve opening time or system pressure. Also, the non-contact liquid dispensing process was investigated with computational fluid dynamics (CFD) models, which make us understand the droplet breakup process in more detail and make dispensing nanoliter droplet fall off successfully without attach to the nozzle. Experiments were carried out with different dispensing volumes, coefficient of variance (CV) has been shown to be below 3% at 1μl and approach 8% at 50 nl.
AB - Recently, high-throughput protein crystallization requires the ability to transfer submicroliter volumes of sample with a large range of viscosities. In this paper, a solenoid-based non-contact liquid handling instrument with high-speed flow sensor was developed to meet this requirement. The flow sensor-driven dispenser with feedback control automatically compensates for encountered differences or changes in sample viscosity by adjusting the valve opening time or system pressure. Also, the non-contact liquid dispensing process was investigated with computational fluid dynamics (CFD) models, which make us understand the droplet breakup process in more detail and make dispensing nanoliter droplet fall off successfully without attach to the nozzle. Experiments were carried out with different dispensing volumes, coefficient of variance (CV) has been shown to be below 3% at 1μl and approach 8% at 50 nl.
KW - Computational fluid dynamics (CFD)
KW - Flow sensor
KW - MEMS
KW - Non-contact liquid dispensing
UR - https://www.scopus.com/pages/publications/52949130275
U2 - 10.1109/NANO.2007.4601396
DO - 10.1109/NANO.2007.4601396
M3 - 会议稿件
AN - SCOPUS:52949130275
SN - 1424406080
SN - 9781424406081
T3 - 2007 7th IEEE International Conference on Nanotechnology - IEEE-NANO 2007, Proceedings
SP - 1189
EP - 1194
BT - 2007 7th IEEE International Conference on Nanotechnology - IEEE-NANO 2007, Proceedings
T2 - 2007 7th IEEE International Conference on Nanotechnology - IEEE-NANO 2007
Y2 - 2 August 2007 through 5 August 2007
ER -