A novel method for rapid detection of class IIa bacteriocin-producing lactic acid bacteria

Huaxi Yi; Lanwei Zhang; Yanfeng Tuo; Xue Han; Ming Du

doi:10.1016/j.foodcont.2009.07.002

A novel method for rapid detection of class IIa bacteriocin-producing lactic acid bacteria

Huaxi Yi
, Lanwei Zhang^*
, Yanfeng Tuo
, Xue Han
, Ming Du

^*Corresponding author for this work

School of Chemistry and Chemical Engineering

Harbin Institute of Technology
Gansu Agricultural University

Research output: Contribution to journal › Article › peer-review

52 Scopus citations

Abstract

A method based on colony PCR was developed for rapid screening of class IIa bacteriocin-producing lactic acid bacteria. The principle of the method is to detect the presence of bacteriocin gene by using specific degenerate primers to amplify a 3 kb fragment from bacteriocin structural gene to histidine kinase gene regions. Degenerate primers were designed according to the conserved amino acid sequences (YGNGVXCXXXXCXV and LDNAIE) contained in class IIa bacteriocins. This assay showed agreement with the conventional well-diffusion method. These findings indicate that the method proposed in this study is considerably more rapid and sufficient for screening of class IIa bacteriocin-producing bacterial isolates in environmental samples. Crown

Original language	English
Pages (from-to)	426-430
Number of pages	5
Journal	Food Control
Volume	21
Issue number	4
DOIs	https://doi.org/10.1016/j.foodcont.2009.07.002
State	Published - Apr 2010

Keywords

Class IIa bacteriocin
Colony PCR
Lactic acid bacteria

Access to Document

10.1016/j.foodcont.2009.07.002

Cite this

@article{aeb3a64e78164d3e87a2535b57534087,

title = "A novel method for rapid detection of class IIa bacteriocin-producing lactic acid bacteria",

abstract = "A method based on colony PCR was developed for rapid screening of class IIa bacteriocin-producing lactic acid bacteria. The principle of the method is to detect the presence of bacteriocin gene by using specific degenerate primers to amplify a 3 kb fragment from bacteriocin structural gene to histidine kinase gene regions. Degenerate primers were designed according to the conserved amino acid sequences (YGNGVXCXXXXCXV and LDNAIE) contained in class IIa bacteriocins. This assay showed agreement with the conventional well-diffusion method. These findings indicate that the method proposed in this study is considerably more rapid and sufficient for screening of class IIa bacteriocin-producing bacterial isolates in environmental samples. Crown",

keywords = "Class IIa bacteriocin, Colony PCR, Lactic acid bacteria",

author = "Huaxi Yi and Lanwei Zhang and Yanfeng Tuo and Xue Han and Ming Du",

year = "2010",

month = apr,

doi = "10.1016/j.foodcont.2009.07.002",

language = "英语",

volume = "21",

pages = "426--430",

journal = "Food Control",

issn = "0956-7135",

publisher = "Elsevier B.V.",

number = "4",

}

TY - JOUR

T1 - A novel method for rapid detection of class IIa bacteriocin-producing lactic acid bacteria

AU - Yi, Huaxi

AU - Zhang, Lanwei

AU - Tuo, Yanfeng

AU - Han, Xue

AU - Du, Ming

PY - 2010/4

Y1 - 2010/4

N2 - A method based on colony PCR was developed for rapid screening of class IIa bacteriocin-producing lactic acid bacteria. The principle of the method is to detect the presence of bacteriocin gene by using specific degenerate primers to amplify a 3 kb fragment from bacteriocin structural gene to histidine kinase gene regions. Degenerate primers were designed according to the conserved amino acid sequences (YGNGVXCXXXXCXV and LDNAIE) contained in class IIa bacteriocins. This assay showed agreement with the conventional well-diffusion method. These findings indicate that the method proposed in this study is considerably more rapid and sufficient for screening of class IIa bacteriocin-producing bacterial isolates in environmental samples. Crown

AB - A method based on colony PCR was developed for rapid screening of class IIa bacteriocin-producing lactic acid bacteria. The principle of the method is to detect the presence of bacteriocin gene by using specific degenerate primers to amplify a 3 kb fragment from bacteriocin structural gene to histidine kinase gene regions. Degenerate primers were designed according to the conserved amino acid sequences (YGNGVXCXXXXCXV and LDNAIE) contained in class IIa bacteriocins. This assay showed agreement with the conventional well-diffusion method. These findings indicate that the method proposed in this study is considerably more rapid and sufficient for screening of class IIa bacteriocin-producing bacterial isolates in environmental samples. Crown

KW - Class IIa bacteriocin

KW - Colony PCR

KW - Lactic acid bacteria

UR - https://www.scopus.com/pages/publications/71549123681

U2 - 10.1016/j.foodcont.2009.07.002

DO - 10.1016/j.foodcont.2009.07.002

M3 - 文章

AN - SCOPUS:71549123681

SN - 0956-7135

VL - 21

SP - 426

EP - 430

JO - Food Control

JF - Food Control

IS - 4

ER -

A novel method for rapid detection of class IIa bacteriocin-producing lactic acid bacteria

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this