A new Cghd i gene with negative regulation on the synthesis of chaetoglobosin A

Cheng Jiang; Jinzhu Song; Junzheng Zhang

doi:10.1088/1757-899X/768/5/052049

A new Cghd i gene with negative regulation on the synthesis of chaetoglobosin A

Cheng Jiang^*
, Jinzhu Song
, Junzheng Zhang

^*Corresponding author for this work

Huainan Normal University
School of Life Science and Technology, Harbin Institute of Technology
School of Chemistry and Chemical Engineering, Harbin Institute of Technology

Research output: Contribution to journal › Conference article › peer-review

Abstract

Chaetoglobosins have wide application prospect, but the low yields make it not widely used. The aim of this study was to obtain high-yielding strains and to find the genes regulating the synthesis of chaetoglobosins. A strain with yield increased to 115% was obtained through inserting a short-DNA randomly into the genome of Chaetomium globosum by agrobacterium. At the same time, inverse PCR was used to determine the insertion position of T-DNA. A new gene Cghd I with negative regulation on the synthesis of chaetoglobosin A was found, indicating that the detectable random insertability mutation technique established in this paper can be used to obtain high yield strains and find regulatory genes.

Original language	English
Article number	052049
Journal	IOP Conference Series: Materials Science and Engineering
Volume	768
Issue number	5
DOIs	https://doi.org/10.1088/1757-899X/768/5/052049
State	Published - 30 Mar 2020
Externally published	Yes
Event	3rd International Symposium on Application of Materials Science and Energy Materials, SAMSE 2019 - Shanghai, China Duration: 30 Dec 2019 → 31 Dec 2019

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10.1088/1757-899X/768/5/052049

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title = "A new Cghd i gene with negative regulation on the synthesis of chaetoglobosin A",

abstract = "Chaetoglobosins have wide application prospect, but the low yields make it not widely used. The aim of this study was to obtain high-yielding strains and to find the genes regulating the synthesis of chaetoglobosins. A strain with yield increased to 115\% was obtained through inserting a short-DNA randomly into the genome of Chaetomium globosum by agrobacterium. At the same time, inverse PCR was used to determine the insertion position of T-DNA. A new gene Cghd I with negative regulation on the synthesis of chaetoglobosin A was found, indicating that the detectable random insertability mutation technique established in this paper can be used to obtain high yield strains and find regulatory genes.",

author = "Cheng Jiang and Jinzhu Song and Junzheng Zhang",

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T1 - A new Cghd i gene with negative regulation on the synthesis of chaetoglobosin A

AU - Jiang, Cheng

AU - Song, Jinzhu

AU - Zhang, Junzheng

PY - 2020/3/30

Y1 - 2020/3/30

N2 - Chaetoglobosins have wide application prospect, but the low yields make it not widely used. The aim of this study was to obtain high-yielding strains and to find the genes regulating the synthesis of chaetoglobosins. A strain with yield increased to 115% was obtained through inserting a short-DNA randomly into the genome of Chaetomium globosum by agrobacterium. At the same time, inverse PCR was used to determine the insertion position of T-DNA. A new gene Cghd I with negative regulation on the synthesis of chaetoglobosin A was found, indicating that the detectable random insertability mutation technique established in this paper can be used to obtain high yield strains and find regulatory genes.

AB - Chaetoglobosins have wide application prospect, but the low yields make it not widely used. The aim of this study was to obtain high-yielding strains and to find the genes regulating the synthesis of chaetoglobosins. A strain with yield increased to 115% was obtained through inserting a short-DNA randomly into the genome of Chaetomium globosum by agrobacterium. At the same time, inverse PCR was used to determine the insertion position of T-DNA. A new gene Cghd I with negative regulation on the synthesis of chaetoglobosin A was found, indicating that the detectable random insertability mutation technique established in this paper can be used to obtain high yield strains and find regulatory genes.

UR - https://www.scopus.com/pages/publications/85083018315

U2 - 10.1088/1757-899X/768/5/052049

DO - 10.1088/1757-899X/768/5/052049

M3 - 会议文章

AN - SCOPUS:85083018315

SN - 1757-8981

VL - 768

JO - IOP Conference Series: Materials Science and Engineering

JF - IOP Conference Series: Materials Science and Engineering

IS - 5

M1 - 052049

T2 - 3rd International Symposium on Application of Materials Science and Energy Materials, SAMSE 2019

Y2 - 30 December 2019 through 31 December 2019

ER -

A new Cghd i gene with negative regulation on the synthesis of chaetoglobosin A

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