A microfluidic array for parallelized single-cell gene expression profiling

H. Sun; T. Olsen; J. Zhu; B. Ponnaiya; S. Amundson; D. Brenner; J. Tao; Q. Lin

A microfluidic array for parallelized single-cell gene expression profiling

H. Sun
, T. Olsen
, J. Zhu
, B. Ponnaiya
, S. Amundson
, D. Brenner
, J. Tao
, Q. Lin^*

^*Corresponding author for this work

School of Mechatronics Engineering

Columbia University

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

The ability to correlate single-cell genetic information with cellular phenotypes holds the potential to interpret disease pathways that is not attainable from bulk analysis. Microfluidics is attractive for single-cell analysis due to precise handling, low risk of contamination, reduced reagent cost and parallelized operation. We present an integrated microfluidic array for parallelized single-cell messenger RNA (mRNA) transcriptional analysis using quantitative reverse transcription polymerase chain reaction (RT-qPCR). The transcriptional levels of individual human cancer cells (MCF-7) were assayed for the induction of CDKN1A using a Taqman probe/primer set in the array.

Original language	English
Title of host publication	18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
Publisher	Chemical and Biological Microsystems Society
Pages	799-801
Number of pages	3
ISBN (Electronic)	9780979806476
State	Published - 2014
Event	18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - San Antonio, United States Duration: 26 Oct 2014 → 30 Oct 2014

Publication series

Name	18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014

Conference

Conference	18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
Country/Territory	United States
City	San Antonio
Period	26/10/14 → 30/10/14

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Microfluidic array
RT-qPCR
Single-cell genetic analysis

Cite this

Sun, H., Olsen, T., Zhu, J., Ponnaiya, B., Amundson, S., Brenner, D., Tao, J., & Lin, Q. (2014). A microfluidic array for parallelized single-cell gene expression profiling. In 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 (pp. 799-801). (18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014). Chemical and Biological Microsystems Society.

Sun, H. ; Olsen, T. ; Zhu, J. et al. / A microfluidic array for parallelized single-cell gene expression profiling. 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society, 2014. pp. 799-801 (18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014).

@inproceedings{ebe8a2a97e86422ab40706991409f0ce,

title = "A microfluidic array for parallelized single-cell gene expression profiling",

abstract = "The ability to correlate single-cell genetic information with cellular phenotypes holds the potential to interpret disease pathways that is not attainable from bulk analysis. Microfluidics is attractive for single-cell analysis due to precise handling, low risk of contamination, reduced reagent cost and parallelized operation. We present an integrated microfluidic array for parallelized single-cell messenger RNA (mRNA) transcriptional analysis using quantitative reverse transcription polymerase chain reaction (RT-qPCR). The transcriptional levels of individual human cancer cells (MCF-7) were assayed for the induction of CDKN1A using a Taqman probe/primer set in the array.",

keywords = "Microfluidic array, RT-qPCR, Single-cell genetic analysis",

author = "H. Sun and T. Olsen and J. Zhu and B. Ponnaiya and S. Amundson and D. Brenner and J. Tao and Q. Lin",

note = "Publisher Copyright: {\textcopyright} 14CBMS.; 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 ; Conference date: 26-10-2014 Through 30-10-2014",

year = "2014",

language = "英语",

series = "18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014",

publisher = "Chemical and Biological Microsystems Society",

pages = "799--801",

booktitle = "18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014",

}

Sun, H, Olsen, T, Zhu, J, Ponnaiya, B, Amundson, S, Brenner, D, Tao, J & Lin, Q 2014, A microfluidic array for parallelized single-cell gene expression profiling. in 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014, Chemical and Biological Microsystems Society, pp. 799-801, 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014, San Antonio, United States, 26/10/14.

A microfluidic array for parallelized single-cell gene expression profiling. / Sun, H.; Olsen, T.; Zhu, J. et al.
18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society, 2014. p. 799-801 (18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - A microfluidic array for parallelized single-cell gene expression profiling

AU - Sun, H.

AU - Olsen, T.

AU - Zhu, J.

AU - Ponnaiya, B.

AU - Amundson, S.

AU - Brenner, D.

AU - Tao, J.

AU - Lin, Q.

PY - 2014

Y1 - 2014

N2 - The ability to correlate single-cell genetic information with cellular phenotypes holds the potential to interpret disease pathways that is not attainable from bulk analysis. Microfluidics is attractive for single-cell analysis due to precise handling, low risk of contamination, reduced reagent cost and parallelized operation. We present an integrated microfluidic array for parallelized single-cell messenger RNA (mRNA) transcriptional analysis using quantitative reverse transcription polymerase chain reaction (RT-qPCR). The transcriptional levels of individual human cancer cells (MCF-7) were assayed for the induction of CDKN1A using a Taqman probe/primer set in the array.

AB - The ability to correlate single-cell genetic information with cellular phenotypes holds the potential to interpret disease pathways that is not attainable from bulk analysis. Microfluidics is attractive for single-cell analysis due to precise handling, low risk of contamination, reduced reagent cost and parallelized operation. We present an integrated microfluidic array for parallelized single-cell messenger RNA (mRNA) transcriptional analysis using quantitative reverse transcription polymerase chain reaction (RT-qPCR). The transcriptional levels of individual human cancer cells (MCF-7) were assayed for the induction of CDKN1A using a Taqman probe/primer set in the array.

KW - Microfluidic array

KW - RT-qPCR

KW - Single-cell genetic analysis

UR - https://www.scopus.com/pages/publications/84941645096

M3 - 会议稿件

AN - SCOPUS:84941645096

T3 - 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014

SP - 799

EP - 801

BT - 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014

PB - Chemical and Biological Microsystems Society

T2 - 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014

Y2 - 26 October 2014 through 30 October 2014

ER -

Sun H, Olsen T, Zhu J, Ponnaiya B, Amundson S, Brenner D et al. A microfluidic array for parallelized single-cell gene expression profiling. In 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society. 2014. p. 799-801. (18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014).

A microfluidic array for parallelized single-cell gene expression profiling

Abstract

Publication series

Conference

UN SDGs

Keywords

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