A highly selective AIE fluorogen for lipid droplet imaging in live cells and green algae

Erjing Wang; Engui Zhao; Yuning Hong; Jacky W.Y. Lam; Ben Zhong Tang

doi:10.1039/c3tb21675f

A highly selective AIE fluorogen for lipid droplet imaging in live cells and green algae

Erjing Wang
, Engui Zhao
, Yuning Hong
, Jacky W.Y. Lam
, Ben Zhong Tang^*

^*Corresponding author for this work

Hong Kong University of Science and Technology
South China University of Technology

Research output: Contribution to journal › Article › peer-review

121 Scopus citations

Abstract

Lipid droplets (LDs) are subcellular organelles for energy storage and lipid metabolism regulation. Here we report an aggregation-induced emission-active fluorogen, TPE-AmAl, for specific LD imaging. TPE-AmAl is cell-permeable: upon entering the live cells, the dye molecules can selectively accumulate in the LDs and turn on the fluorescence. TPE-AmAl possesses twisted intramolecular charge transfer properties as well: the emission colour in the hydrophobic LDs is blue-shifted by >100 nm than that in aqueous buffers. Compared with the commercial lipid droplet dye, TPE-AmAl demonstrates the advantages of low background, short staining time, high selectivity, excellent biocompatibility, and good photostability. The utilization of TPE-AmAl for LD staining in green algae is also demonstrated, indicating their potential application in the high-throughput screening of high-value microalgae as a preferential biofuel source. This journal is

Original language	English
Pages (from-to)	2013-2019
Number of pages	7
Journal	Journal of Materials Chemistry B
Volume	2
Issue number	14
DOIs	https://doi.org/10.1039/c3tb21675f
State	Published - 14 Apr 2014
Externally published	Yes

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title = "A highly selective AIE fluorogen for lipid droplet imaging in live cells and green algae",

abstract = "Lipid droplets (LDs) are subcellular organelles for energy storage and lipid metabolism regulation. Here we report an aggregation-induced emission-active fluorogen, TPE-AmAl, for specific LD imaging. TPE-AmAl is cell-permeable: upon entering the live cells, the dye molecules can selectively accumulate in the LDs and turn on the fluorescence. TPE-AmAl possesses twisted intramolecular charge transfer properties as well: the emission colour in the hydrophobic LDs is blue-shifted by >100 nm than that in aqueous buffers. Compared with the commercial lipid droplet dye, TPE-AmAl demonstrates the advantages of low background, short staining time, high selectivity, excellent biocompatibility, and good photostability. The utilization of TPE-AmAl for LD staining in green algae is also demonstrated, indicating their potential application in the high-throughput screening of high-value microalgae as a preferential biofuel source. This journal is",

author = "Erjing Wang and Engui Zhao and Yuning Hong and Lam, \{Jacky W.Y.\} and Tang, \{Ben Zhong\}",

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doi = "10.1039/c3tb21675f",

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T1 - A highly selective AIE fluorogen for lipid droplet imaging in live cells and green algae

AU - Wang, Erjing

AU - Zhao, Engui

AU - Hong, Yuning

AU - Lam, Jacky W.Y.

AU - Tang, Ben Zhong

PY - 2014/4/14

Y1 - 2014/4/14

N2 - Lipid droplets (LDs) are subcellular organelles for energy storage and lipid metabolism regulation. Here we report an aggregation-induced emission-active fluorogen, TPE-AmAl, for specific LD imaging. TPE-AmAl is cell-permeable: upon entering the live cells, the dye molecules can selectively accumulate in the LDs and turn on the fluorescence. TPE-AmAl possesses twisted intramolecular charge transfer properties as well: the emission colour in the hydrophobic LDs is blue-shifted by >100 nm than that in aqueous buffers. Compared with the commercial lipid droplet dye, TPE-AmAl demonstrates the advantages of low background, short staining time, high selectivity, excellent biocompatibility, and good photostability. The utilization of TPE-AmAl for LD staining in green algae is also demonstrated, indicating their potential application in the high-throughput screening of high-value microalgae as a preferential biofuel source. This journal is

AB - Lipid droplets (LDs) are subcellular organelles for energy storage and lipid metabolism regulation. Here we report an aggregation-induced emission-active fluorogen, TPE-AmAl, for specific LD imaging. TPE-AmAl is cell-permeable: upon entering the live cells, the dye molecules can selectively accumulate in the LDs and turn on the fluorescence. TPE-AmAl possesses twisted intramolecular charge transfer properties as well: the emission colour in the hydrophobic LDs is blue-shifted by >100 nm than that in aqueous buffers. Compared with the commercial lipid droplet dye, TPE-AmAl demonstrates the advantages of low background, short staining time, high selectivity, excellent biocompatibility, and good photostability. The utilization of TPE-AmAl for LD staining in green algae is also demonstrated, indicating their potential application in the high-throughput screening of high-value microalgae as a preferential biofuel source. This journal is

UR - https://www.scopus.com/pages/publications/84896105000

U2 - 10.1039/c3tb21675f

DO - 10.1039/c3tb21675f

M3 - 文章

AN - SCOPUS:84896105000

SN - 2050-750X

VL - 2

SP - 2013

EP - 2019

JO - Journal of Materials Chemistry B

JF - Journal of Materials Chemistry B

IS - 14

ER -

A highly selective AIE fluorogen for lipid droplet imaging in live cells and green algae

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