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A DEP-assisted single-cell electroporation chip with low operation voltage

  • Jinpeng Wang*
  • , Shih Chi Yang
  • , Cheng Wang
  • , Qiong Wu
  • , Zheyao Wang
  • *Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

This paper reports an insulator-based dielectrophoresis (iDEP)-assisted single-cell electroporation chip that can locally electroporate cells with an operation voltage of 1V. The electroporation chip consists of two metal electrodes and a sandwiched dielectric polymer layer with microwells. The dielectric microwell arrays create non-uniform electric fields to generate positive DEP forces to capture a single-cell at each microwell. The following DC pulse voltage imposes a large electric field across the membranes of the captured cells and electroporates the cells. Human lymphoma cells in an 8 x 11 array are successfully electroporated.

Original languageEnglish
Title of host publicationIEEE Sensors 2010 Conference, SENSORS 2010
Pages2097-2100
Number of pages4
DOIs
StatePublished - 2010
Externally publishedYes
Event9th IEEE Sensors Conference 2010, SENSORS 2010 - Waikoloa, HI, United States
Duration: 1 Nov 20104 Nov 2010

Publication series

NameProceedings of IEEE Sensors

Conference

Conference9th IEEE Sensors Conference 2010, SENSORS 2010
Country/TerritoryUnited States
CityWaikoloa, HI
Period1/11/104/11/10

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