A Coumarin-Based Reversible Fluorescent Probe for Quantitative Detection and Monitoring of Cellular Glutathione

Shanshan Wang; Shihua Yu; Zhigang Liu; Xiaodan Zeng

doi:10.1002/slct.202500218

A Coumarin-Based Reversible Fluorescent Probe for Quantitative Detection and Monitoring of Cellular Glutathione

Shanshan Wang
, Shihua Yu
, Zhigang Liu^*
, Xiaodan Zeng^*

^*Corresponding author for this work

Jilin Institute of Chemical Technology

Research output: Contribution to journal › Article › peer-review

Abstract

An “on-off-on” reversible fluorescent probe for reversible response to GSH and H₂O₂ and determination for GSH was designed and synthesized. Considering the important role of GSH in biological system and commonly applied recognition groups, the C═C bond in coumarin dye was successfully used as the recognition site. This probe showed fast response (within 5 s) and high sensitivity (12.6 nM) in the recognition process. It could be used not only for quantitative determination of GSH, but also could be used for the fluorescence imaging of GSH in cells. In addition, the probe showed reversible fluorescence response for GSH and H₂O₂, which could be repeated at least 3 times. The experimental results proved that the probe was successfully modified from coumarin and the probe recognized for GSH with significant results.

Original language	English
Article number	e202500218
Journal	ChemistrySelect
Volume	10
Issue number	12
DOIs	https://doi.org/10.1002/slct.202500218
State	Published - 26 Mar 2025
Externally published	Yes

Keywords

Coumarin
Fluorescence imaging
Glutathione
Reversible fluorescent probe

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10.1002/slct.202500218

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title = "A Coumarin-Based Reversible Fluorescent Probe for Quantitative Detection and Monitoring of Cellular Glutathione",

abstract = "An {\textquotedblleft}on-off-on{\textquotedblright} reversible fluorescent probe for reversible response to GSH and H2O2 and determination for GSH was designed and synthesized. Considering the important role of GSH in biological system and commonly applied recognition groups, the C═C bond in coumarin dye was successfully used as the recognition site. This probe showed fast response (within 5 s) and high sensitivity (12.6 nM) in the recognition process. It could be used not only for quantitative determination of GSH, but also could be used for the fluorescence imaging of GSH in cells. In addition, the probe showed reversible fluorescence response for GSH and H2O2, which could be repeated at least 3 times. The experimental results proved that the probe was successfully modified from coumarin and the probe recognized for GSH with significant results.",

keywords = "Coumarin, Fluorescence imaging, Glutathione, Reversible fluorescent probe",

author = "Shanshan Wang and Shihua Yu and Zhigang Liu and Xiaodan Zeng",

note = "Publisher Copyright: {\textcopyright} 2025 Wiley-VCH GmbH.",

year = "2025",

month = mar,

day = "26",

doi = "10.1002/slct.202500218",

language = "英语",

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TY - JOUR

T1 - A Coumarin-Based Reversible Fluorescent Probe for Quantitative Detection and Monitoring of Cellular Glutathione

AU - Wang, Shanshan

AU - Yu, Shihua

AU - Liu, Zhigang

AU - Zeng, Xiaodan

PY - 2025/3/26

Y1 - 2025/3/26

N2 - An “on-off-on” reversible fluorescent probe for reversible response to GSH and H2O2 and determination for GSH was designed and synthesized. Considering the important role of GSH in biological system and commonly applied recognition groups, the C═C bond in coumarin dye was successfully used as the recognition site. This probe showed fast response (within 5 s) and high sensitivity (12.6 nM) in the recognition process. It could be used not only for quantitative determination of GSH, but also could be used for the fluorescence imaging of GSH in cells. In addition, the probe showed reversible fluorescence response for GSH and H2O2, which could be repeated at least 3 times. The experimental results proved that the probe was successfully modified from coumarin and the probe recognized for GSH with significant results.

AB - An “on-off-on” reversible fluorescent probe for reversible response to GSH and H2O2 and determination for GSH was designed and synthesized. Considering the important role of GSH in biological system and commonly applied recognition groups, the C═C bond in coumarin dye was successfully used as the recognition site. This probe showed fast response (within 5 s) and high sensitivity (12.6 nM) in the recognition process. It could be used not only for quantitative determination of GSH, but also could be used for the fluorescence imaging of GSH in cells. In addition, the probe showed reversible fluorescence response for GSH and H2O2, which could be repeated at least 3 times. The experimental results proved that the probe was successfully modified from coumarin and the probe recognized for GSH with significant results.

KW - Coumarin

KW - Fluorescence imaging

KW - Glutathione

KW - Reversible fluorescent probe

UR - https://www.scopus.com/pages/publications/105000812794

U2 - 10.1002/slct.202500218

DO - 10.1002/slct.202500218

M3 - 文章

AN - SCOPUS:105000812794

SN - 2365-6549

VL - 10

JO - ChemistrySelect

JF - ChemistrySelect

IS - 12

M1 - e202500218

ER -

A Coumarin-Based Reversible Fluorescent Probe for Quantitative Detection and Monitoring of Cellular Glutathione

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Keywords

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