面向非生物学专业的 CRISPR/Cas9 基因编辑实验教学的设计与实践

Ning Gu; Youpeng Qu; Ruijiao Zhu; Changlin Wang; Jinzhu Song; Fang Han

doi:10.16791/j.cnki.sjg.2023.04.028

面向非生物学专业的 CRISPR/Cas9 基因编辑实验教学的设计与实践

Translated title of the contribution: Design and practice of CRISPR/Cas9 gene editing experiment teaching for non-biological majors

Ning Gu
, Youpeng Qu
, Ruijiao Zhu
, Changlin Wang
, Jinzhu Song
, Fang Han^*

^*Corresponding author for this work

School of Life Science and Technology, Harbin Institute of Technology

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Based on CRISPR/Cas9 gene editing technology, an experimental course of “Using gene editing technology to establish target gene knockout cell lines” was designed for non-biological students. The experiment includes confirmatory and exploratory content, integrating multidisciplinary knowledge points such as bioinformatics, molecular biology, cell biology, genetic engineering. It is conducive to strengthening students' understanding of relevant life science knowledge, improving their comprehensive experimental design ability and cultivating their innovative spirit.

Translated title of the contribution	Design and practice of CRISPR/Cas9 gene editing experiment teaching for non-biological majors
Original language	Chinese (Traditional)
Pages (from-to)	187-191 and 221
Journal	Experimental Technology and Management
Volume	40
Issue number	4
DOIs	https://doi.org/10.16791/j.cnki.sjg.2023.04.028
State	Published - Apr 2023
Externally published	Yes

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Cite this

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title = "面向非生物学专业的 CRISPR/Cas9 基因编辑实验教学的设计与实践",

abstract = "Based on CRISPR/Cas9 gene editing technology, an experimental course of “Using gene editing technology to establish target gene knockout cell lines” was designed for non-biological students. The experiment includes confirmatory and exploratory content, integrating multidisciplinary knowledge points such as bioinformatics, molecular biology, cell biology, genetic engineering. It is conducive to strengthening students' understanding of relevant life science knowledge, improving their comprehensive experimental design ability and cultivating their innovative spirit.",

keywords = "CRISPR/Cas9, gene editing, innovative experiments, teaching reform",

author = "Ning Gu and Youpeng Qu and Ruijiao Zhu and Changlin Wang and Jinzhu Song and Fang Han",

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AU - Gu, Ning

AU - Qu, Youpeng

AU - Zhu, Ruijiao

AU - Wang, Changlin

AU - Song, Jinzhu

AU - Han, Fang

PY - 2023/4

Y1 - 2023/4

N2 - Based on CRISPR/Cas9 gene editing technology, an experimental course of “Using gene editing technology to establish target gene knockout cell lines” was designed for non-biological students. The experiment includes confirmatory and exploratory content, integrating multidisciplinary knowledge points such as bioinformatics, molecular biology, cell biology, genetic engineering. It is conducive to strengthening students' understanding of relevant life science knowledge, improving their comprehensive experimental design ability and cultivating their innovative spirit.

AB - Based on CRISPR/Cas9 gene editing technology, an experimental course of “Using gene editing technology to establish target gene knockout cell lines” was designed for non-biological students. The experiment includes confirmatory and exploratory content, integrating multidisciplinary knowledge points such as bioinformatics, molecular biology, cell biology, genetic engineering. It is conducive to strengthening students' understanding of relevant life science knowledge, improving their comprehensive experimental design ability and cultivating their innovative spirit.

KW - CRISPR/Cas9

KW - gene editing

KW - innovative experiments

KW - teaching reform

UR - https://www.scopus.com/pages/publications/105021960041

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DO - 10.16791/j.cnki.sjg.2023.04.028

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ER -

面向非生物学专业的 CRISPR/Cas9 基因编辑实验教学的设计与实践

Abstract

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