腐殖酸和产甲烷抑制剂对厌氧污泥发酵产氢效能的影响

To improve the hydrogen production capacity of anaerobic sludge, the effects of humic acid combining with different methanogenesis inhibitors on hydrogen production from glucose were investigated by batch culture. The shift of microbial community structure was also revealed by Illumina MiSeq sequencing. The results showed that the cumulative hydrogen production in control was 1.2 mL after 120 h cultivation under the initial glucose concentration of 500 mg•L^-1 condition. In addition, an obvious hydrogen consumption phenomenon could be observed in control. When chloroform of 0.02% (V/V), 0.04% (V/V) or 2-bromoethanesulphonate (10 mmol•L^-1) were added to the fermentation systems, the hydrogen consumption was effectively inhibited. Their cumulative hydrogen production were 15.7, 13.2, and 9.4 mL, respectively. Both methanogenesis inhibitors and humic acid were added to the fermentation systems led to the hydrogen production significantly enhanced. Among them, the maximum efficiency of hydrogen production was obtained in humic acid combining with 0.02% chloroform fermentation system. The cumulative hydrogen production and maximum hydrogen production rate (R_max) in this system reached 17.4 mL and 0.24 mL•L^-1, respectively. On the contrary, when tinidazole was used as methanogenesis inhibitor, hydrogen consumption could still be observed. Illumina MiSeq sequencing showed that there were significant differences in the relative abundance of dominant microbial groups in different fermentation systems. The dominant microbial community were Ottowia, Ignavibacterium, Saccharibacteria_genera_incertae_sedis and Terrimonas with relative abundance of 2.2%~4.2% in control. When 0.02% chloroform and humic acid were added to fermentation system, the relative abundance of Ottowia and Saccharibacteria_genera_incertae_sedis were increased by 11.9% and 31.8%, respectively. However, the number of Nitrospira was significantly reduced.