基于UPLC-Triple-TOF MS/MS对巴氏杀菌乳中磷脂成分的分析

The phospholipids in pasteurized milk samples were analyzed and identified by ultra-high performance liquid chromatography-triple quadrupole-time of flight tandem mass spectrometry (UPLC-Triple-TOF MS/MS). The chromatographic separation was performed on an ACQUITY UPLC^® BEH C₁₈ (2. 1 mm × 100 mm, 1. 7 μm) column using gradient elution with water/methanol/acetonitrile (1:1:1, V/V) as mobile phase A and isopropanol/acetonitrile (1:1, V/V)as mobile phase B. Data acquisition was done in the positive ion mode using electrospray ionization (ESI). According to the high-resolution mass spectral data of retention time, quasi-molecular ions and secondary fragments combined with information on the mass spectral fragmentation pathways of reference standards, a total of 68 phospholipid molecules were identified including 22 phosphatidylcholine (PC, 7.98%), 17 phosphatidylethanolamine (PE, 56.60%), 10 phosphatidylserine(PS, 1.70%), 7 phosphatidylinositol (PI, 1.33%), and 12 sphingomyelin (SM, 32.39%). This study achieved comprehensive qualitative and quantitative determination of phospholipids in milk by using the UPLC-Triple-TOF MS/MS method with high sensitivity, precision and stability. This method could detect more phospholipids in milk than other detection techniques and thus provide more basic data for research on milk phospholipids.