基于SERS技术检测牛奶中氨苄西林的研究

Objective To detect ampicillin in milk using surface enhanced Raman spectroscopy (SERS) and obtain qualitative and semi-quantitative analysis of ampicillin. Methods Firstly, a SERS detection platform of alumina ceramic substrate was constructed. Secondly, Raman characteristic peaks (1000cm^-1) of ampicillin were assigned and the optimal experimental conditions were determined. Then the ampicillin in water was detected using SERS and the limit of detection was obtained. Finally, the ampicillin in milk was detected and the semi-quantitative analysis of spiked milk samples of ampicillin was achieved using linear fitting. Results The limit of detection of ampicillin in milk samples is 1.0×10^-3g/L. In the range of concentrations of 10^-3~10^-1g/L, the relationship of Raman peak intensity and concentration of ampicillin in milk can be expressed by a linear equation: y=23408.93x+1801.47(R²=0.993).The recovery rate was detected between 95.0% and 110.0%. Conclusion The SERS detection process is less than seven minutes, including the pretreatment process of milk samples (about five minutes). This method has great potential for the quantitative and qualitative analysis of pollutants in milk.