基于人工神经网络和遗传算法的普鲁兰酶重组大肠杆菌高密度发酵工艺优化

In this study, the high cell density cultivation of recombinant Escherichia coli BL 21 for the production of a novel thermostable pullulanase was optimized using artificial neural network and genetic algorithm. The effects of culture temperature, medium pH, and carbon-to-nitrogen (C/N) molar ratio were tested in a 5 L bioreactor. The results suggested that the optimal culture conditions before the induction phase were as follows: temperature 34.4 ℃, pH 6.87 and C/N ratio 6.1, and the optimal culture conditions after induction were 32.5 ℃, pH 6.69 and 5.3 C/N ratio. The maximum biomass, protein concentration and pullulanase activity obtained under these conditions were 56.5 g/L, 3.21 g/L and 268.3 U/mL, respectively.