人造细胞内蛋白质合成的研究进展

Jing Jing Zhao; Xue Jing Wang; Wei Mu; Xiao Jun Han

doi:10.16476/j.pibb.2020.0163

人造细胞内蛋白质合成的研究进展

Translated title of the contribution: Progress on cell-free protein synthesis inside artificial cells

Jing Jing Zhao
, Xue Jing Wang
, Wei Mu^*
, Xiao Jun Han^*

^*Corresponding author for this work

School of Chemistry and Chemical Engineering, Harbin Institute of Technology

Research output: Contribution to journal › Review article › peer-review

Abstract

Cell-free protein synthesis is an in-vitro cell-free expression method for protein synthesis. High-throughput protein expression and in-vitro reconstitution of membrane proteins can be realized in CFPS-containing artificial cells. This review describes various CFPS systems including E. coli extracts, rabbit reticulocytes extracts, wheat germ extracts, and yeast extracts, and summarizes the progress of cell free protein synthesis inside artificial cells. The challenges and the future directions of this field are also proposed at the end of this paper.

Translated title of the contribution	Progress on cell-free protein synthesis inside artificial cells
Original language	Chinese (Traditional)
Pages (from-to)	121-134
Number of pages	14
Journal	Progress in Biochemistry and Biophysics
Volume	48
Issue number	2
DOIs	https://doi.org/10.16476/j.pibb.2020.0163
State	Published - 2021
Externally published	Yes

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10.16476/j.pibb.2020.0163

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title = "人造细胞内蛋白质合成的研究进展",

abstract = "Cell-free protein synthesis is an in-vitro cell-free expression method for protein synthesis. High-throughput protein expression and in-vitro reconstitution of membrane proteins can be realized in CFPS-containing artificial cells. This review describes various CFPS systems including E. coli extracts, rabbit reticulocytes extracts, wheat germ extracts, and yeast extracts, and summarizes the progress of cell free protein synthesis inside artificial cells. The challenges and the future directions of this field are also proposed at the end of this paper.",

keywords = "Cell-free protein synthesis, Lipid vesicle based artificial cells, Polymersome based artificial cells, Proteinsome based artificial cells",

author = "Zhao, \{Jing Jing\} and Wang, \{Xue Jing\} and Wei Mu and Han, \{Xiao Jun\}",

year = "2021",

doi = "10.16476/j.pibb.2020.0163",

language = "繁体中文",

volume = "48",

pages = "121--134",

journal = "Progress in Biochemistry and Biophysics",

issn = "1000-3282",

publisher = "Science Press ",

number = "2",

}

TY - JOUR

T1 - 人造细胞内蛋白质合成的研究进展

AU - Zhao, Jing Jing

AU - Wang, Xue Jing

AU - Mu, Wei

AU - Han, Xiao Jun

PY - 2021

Y1 - 2021

N2 - Cell-free protein synthesis is an in-vitro cell-free expression method for protein synthesis. High-throughput protein expression and in-vitro reconstitution of membrane proteins can be realized in CFPS-containing artificial cells. This review describes various CFPS systems including E. coli extracts, rabbit reticulocytes extracts, wheat germ extracts, and yeast extracts, and summarizes the progress of cell free protein synthesis inside artificial cells. The challenges and the future directions of this field are also proposed at the end of this paper.

AB - Cell-free protein synthesis is an in-vitro cell-free expression method for protein synthesis. High-throughput protein expression and in-vitro reconstitution of membrane proteins can be realized in CFPS-containing artificial cells. This review describes various CFPS systems including E. coli extracts, rabbit reticulocytes extracts, wheat germ extracts, and yeast extracts, and summarizes the progress of cell free protein synthesis inside artificial cells. The challenges and the future directions of this field are also proposed at the end of this paper.

KW - Cell-free protein synthesis

KW - Lipid vesicle based artificial cells

KW - Polymersome based artificial cells

KW - Proteinsome based artificial cells

UR - https://www.scopus.com/pages/publications/85105943353

U2 - 10.16476/j.pibb.2020.0163

DO - 10.16476/j.pibb.2020.0163

M3 - 文献综述

AN - SCOPUS:85105943353

SN - 1000-3282

VL - 48

SP - 121

EP - 134

JO - Progress in Biochemistry and Biophysics

JF - Progress in Biochemistry and Biophysics

IS - 2

ER -

人造细胞内蛋白质合成的研究进展

Abstract

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