不同防污性能的纳米涂层对海水生物膜中真核菌群的影响

Low surface energy composite antifouling coatings prepared from carbon nanotubes (CNTs) and polydimethylsiloxane (PDMS) have differential antifouling properties; however, few studies have been undertaken on the structure and function of the eukaryotic flora on the coating surfaces that have different antifouling capacities. Nano-filled coatings were prepared using both CNTs and PDMS. Early biofilm samples were collected from marine hanging plates that had good and poor antifouling scores. Biofilm metagenome DNA was extracted and analyzed by high-throughput sequencing of the 18S rRNA V4 region to study the composition of the eukaryotic community, especially the alpha and beta diversity indexes, richness indexes, abundance of operational taxonomic units, and correlation between flora composition and CNTs parameters/environmental factors. During this 24-day study, the structural composition of the eukaryotic flora on the coating surfaces changed greatly; however, the diversity and richness index values of the two types of coatings were similar. The changing trend of the diversity and richness indexes was increase-decrease- increase again for coatings with high antifouling scores, and increase-decrease for coatings with low antifouling scores. The content of Leptothecata was several-fold to 10-fold lower in the coatings with better antifouling performance than in those with poor antifouling performance. The compositional structure of eukaryotic flora on the surface of all CNTs-PDMS coatings changed significantly during the field test period. The two types of antifouling coatings with differential antifouling performance had similar eukaryotic flora structures but the changing trends of the flora were different. These preliminary observations should be investigated further in future studies.